A: They are suitable because they are located within plasmid DNA which can be replicated each time the cell divides which results in man copies of the same gene.
Q: What are "sticky ends"?
A: These are single-stranded regions with unpaired bases that can hydrogen-bond to the complementary sticky ends of fragments made by restriction enzymes.
Q: What is DNA profiling?
A: This the analysis of DNA fragments to determine whether they come from a particular individual or not.
Five main facts:
1) Enzymes are used to essentially "cut and paste" DNA.
2) Cloned genes are able to be stored in a genomic library.
3) Nucleic acid probes help identify clones carrying certain genes.
4) The PCR method is used to amplify DNA sequences.
5) DNA profiling has aided in many investigations by use of forensics.
This depicts the PCR method. This method is used when the source of DNA is scanty or impure. IT allows for a specific gene segment to be targeted and amplified.
This chapter focuses on DNA technology. There are several ways in which this is applied. One is through the research on cloning. Other ways include genetically modified organisms, for example the corn many of us eat. It had been modified genetically to be more resistant to certain diseases and to produce more corn itself. DNA profiling has aided in many forensic cases.
Key Terms:
1) Plasmids: small, circular DNA molecules that replicate separately from the much larger bacterial chromosome
2) Gene cloning: production of multiple identical copes of a gene-carrying piece of DNA
3) Genetic engineering: branch of biotechnology that involves the direct manipulation of genes for practical purposes
4) Vector: gene carrier
5) Clone: group of identical cells descended from a single ancestral cell
6) Genomic library: entire collection of all the cloned DNA fragments from a genome
7) Nucleic acid probe: complementary molecule
8) Vaccine: harmless variant or derivative of a pathogen that is used to stimulate the immune system to mount a defense against that pathogen
9) Gene therapy: alteration of an afflicted individual's genes
10) Primers: short, chemically synthesized single-stranded DNA molecules with sequences that are complementary to one strand at one end of the target sequence
http://www.youtube.com/watch?v=eEcy9k_KsDI
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